If you have a magnetic pickup in the sound hole, the EBow should respond as it would on a solid body electric, though the strings tend to decay more quickly and activate more slowly. Acoustics with bridge transducers, piezo pickups or microphones will be much quieter and, of course, there is no HotSpot, which is necessary for bowing strokes and spiccato. However, you can get a very clean, natural sounding string tone. When moving from string-to-string on an acoustic without a magnetic pickup in the sound hole, you should mute the SupportStrings to reduce the noise of the EBow on the strings. Click here to view YouTube videos featuring EBow on acoustic guitar.